Development and characterization of polymorphic microsatellite loci in the sea cucumber Holothuria leucospilota

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Screening and characterization of novel polymorphic microsatellite markers from sea cucumber Holothuria leucospilota.

The sea cucumber Holothuria leucospilota has high me-dicinal value and rich nutritional edible value, and thus is a commercially important aquatic product in China. Microsatellite loci were developed and screened using a fast isolation protocol and amplified fragment length polymorphism of sequences containing repeats. In this study, 16 novel polymorphic microsatellite markers in H. leucospilot...

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The sea cucumber Holothuria scabra is an endangered species. In this study, nine new polymorphic microsatellite loci were developed and tested in 30 individuals. The number of alleles ranged from 2 to 5, and the observed and expected heterozygosities ranged from 0.1200 to 0.7391 and from 0.2408 to 0.5983, respectively. No loci significantly deviated from the Hardy-Weinberg equilibrium af-ter a ...

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Antibacterial activity of the sea cucumber Holothuria leucospilota

Aquatics are a source of bioactive compounds that these compounds have different properties such as antimicrobial activity. In this study, antibacterial activity of methanol, chloroform and hexane extracts from body wall, gonad and intestine of sea cucumber Holothuria leucospilota was evaluated against Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus. Bioactive compounds of b...

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Diversity and function of aerobic culturable bacteria in the intestine of the sea cucumber Holothuria leucospilota.

Sea cucumbers play an important role in nutrient cycling of marine ecosystems by consuming sediments and moving sand, thus occupying a similar niche to earthworms in terrestrial ecosystems. However, our understanding of microbial diversity and functions associated with sea cucumbers is meager. Here, we isolated 141 bacterial strains under aerobic conditions using various media from the intestin...

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Antioxidant activity of extracts of two species of Sea Cucumber Holothuria parva and Holothuria leucospilota from the Persian Gulf, Iran

Marine animals are among the most important sources of natural products with biological activities because of their secondary metabolites. Many compounds were extracted, purified, identified and studied for their antioxidant, cytotoxic, anti-inflammatory and antibacterial effects from sea cucumber However, very few investigations are available on Persian Gulf sea cucumbers. The antioxidant act...

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ژورنال

عنوان ژورنال: Genetics and Molecular Research

سال: 2015

ISSN: 1676-5680

DOI: 10.4238/2015.january.26.8